### General information

Author: Urska Rovsnik
Contact e-mail: urska.rovsnik@scilifelab.se
DOI: 10.17044/scilifelab.21908892
License: CC BY 4.0
This readme file was last updated: 2023-01-18

### Dataset description

This Dataset contains cryo-electron microscopy (cryo-EM) densities and a pdb model of a gloeobacter violaceus ligand-gated ion channel (GLIC). The densities were generated with RELION software and the model was built with COOT. The dataset also contains a validation report generated by PDB/EMDB deposition where the data has been uploaded and is currently on hold until publication of the paper (https://deposit-pdbe.wwpdb.org/deposition).

The densities, the model and the validation report were requested by revievers for a publication titled 'Discovery of lipid binding sites in a ligand-gated ion channel by integrating simulations and cryo-EM' currently under review at eLife.

Data contains the following items:

1. D_1292124441_val-report-full_P1_4_final.pdf;
	A Validation Report generated for a EMDB/PDB deposition of GLIC  		densities and model.

2. GLIC_popc_220914_final_deposit.pdb;
	A coordinate model of GLIC and lipids
	
3. mask.mrc;
	Soft mask used for local sharpenning of the cryo-EM density
	
4. Phenix_autosharpen_map.mrc;
	Sharpened map used for building of the lipids
	
5. postprocess_masked.mrc;
	Relion postprocessed map
	
6. run_class001.mrc;
	Relion 3D refined map

7. run_half1_class001_unfil.mrc, run_half2_class001_unfil.mrc;
	Relion halfmaps generated by 3D auto refine