Stamping device Muntjewerff et al bio-protocols 2023.stl
STL-file for 3D-printing a stamping device for the creation of a co-culture model designed to fit pancreatic islets, neurons, and immune cells.
The .stl file provides instructions for a 3D printer to print a mold which can be used to stamp out an indentation pattern in a low-melt agarose gel in a standard 6-well plate. The center hole is used for placing one or more pancreatic islets, and the outer crescents are used for placing immune cells by pipetting a cell suspension into the loading ports. The device can be used in this way, or with the addition of a cover slip containing attached neurons placed on top of the gel. This allows for immune cells to migrate, and for neurons to grow into the gel.
For creating the device, pour low-melt agarose into a 6-well plate. Allow the agarose to almost set, and then place the stamping device in each well. The device should leave indentations in the gel. Seal the plate and allow to fully set at 4 deg C before using for cell culture.
Accompanying information and methodology published in:
Three-dimensional Co-culture Model for Live Imaging of Pancreatic Islets, Immune Cells, and Neurons in Agarose Gel
Elke M. Muntjewerff, Vijay S. Josyula, Gustaf Christoffersson
Bio-protocol, Vol 13, Iss 20, Oct 20, 2023. DOI: https://doi.org/10.21769/BioProtoc.4852