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Dots_3Dcoordinates_iFISH_chr1.csv (1.05 MB)
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EXTRACTED_FEATURES.zip (829.51 MB)
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README.txt (2.35 kB)
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beads_chromatic_correction.txt (5.03 kB)
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Simultaneous visualization of DNA loci in single cells by combinatorial multi-color iFISH (Dataset 5: iFISH chr1 spotting Replicate 1)
Version 2 2021-10-25, 06:26
Version 1 2021-06-18, 10:03
figure
posted on 2021-10-25, 06:26 authored by Ana Faustino MotaAna Faustino Mota, Maud Schweitzer, Magda BienkoWe designed 23 probes targeting 23 consecutive loci on chromosome 1 using iFISH4U. The 23 loci are spaced with 10 Mb. There are 3 probes with AF488 dye, 4 probes with AT542, 4 probes with AF594, 4 probes with AT647N, 4 probes with AF700 and 4 probes with AF790. Replicate 1
Experiment Imaging Method: epifluorescence microscopy
Protocol Name: iFISH protocol
Each dataset consists of multiple fields of view (FOVs) that were acquired with seven different dyes (AF488 labeled as ‘a488’; AT542 as ‘tmr’; AF594 as ‘a594’; AT647N as ‘Cy5’, AF700 as ‘a700’, AF790 as ‘ir800’; and Hoechst 33342 as ‘dapi’) and all the FOVs are provided as separate .tiff files. Each FOV comprises 81–95 focal planes spaced 0.2 μm apart and has a size of 1024x1024 pixels.
Protocol Name: iFISH protocol
Each dataset consists of multiple fields of view (FOVs) that were acquired with seven different dyes (AF488 labeled as ‘a488’; AT542 as ‘tmr’; AF594 as ‘a594’; AT647N as ‘Cy5’, AF700 as ‘a700’, AF790 as ‘ir800’; and Hoechst 33342 as ‘dapi’) and all the FOVs are provided as separate .tiff files. Each FOV comprises 81–95 focal planes spaced 0.2 μm apart and has a size of 1024x1024 pixels.
