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Spatiotemporally controlled generation of NTPs for single-molecule studies of NTP-driven processes

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posted on 2022-08-02, 14:05 authored by Anton SabantcevAnton Sabantcev, Guanzhong Mao, Javier Aguirre Rivera, Mikhail Panfilov, Anatolii Arseniev, Oanh Ho, Mikhail A. Khodorkovskiy, Sebastian Deindl
<p>Data supporting the findings of the paper titled "Spatiotemporally controlled generation of NTPs for single-molecule studies" in Nature Chemical Biology which describes a method for controlling NTP-driven reactions in single-molecule experiments <em>via</em> the local generation of NTPs (LAGOON) that increases the measurement throughput and enables single-turnover observations. </p> <p>The dataset contains single-molecule FRET data capturing T4 helicase DNA unwinding, nucleosome remodeling by Chd1 and the invasion of a nucleosome by the T7 RNA polymerase. Additionally, the dataset contains acoustic force spectroscopy data on E. coli RNA polymerase elongation.</p>

Funding

ERC-StG-714068

KAW/WAF 2019.0306

KAW 2016.0077

Cancerfonden 19 0055 Pj

VR 03534

Priority 2030 075-15-2021-1333

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Publisher

Uppsala University

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