posted on 2022-08-02, 14:05authored byAnton SabantcevAnton Sabantcev, Guanzhong Mao, Javier Aguirre Rivera, Mikhail Panfilov, Anatolii Arseniev, Oanh Ho, Mikhail A. Khodorkovskiy, Sebastian Deindl
<p>Data supporting the findings of the paper titled "Spatiotemporally controlled generation of NTPs for single-molecule studies" in Nature Chemical Biology which describes a method for controlling NTP-driven reactions in single-molecule experiments <em>via</em> the local generation of NTPs (LAGOON) that increases the measurement throughput and enables single-turnover observations. </p>
<p>The dataset contains single-molecule FRET data capturing T4 helicase DNA unwinding, nucleosome remodeling by Chd1 and the invasion of a nucleosome by the T7 RNA polymerase. Additionally, the dataset contains acoustic force spectroscopy data on E. coli RNA polymerase elongation.</p>