File(s) not publicly available

Single cell whole genome sequencing of high hyperdiploid acute lymphoblastic leukemia

posted on 2022-02-22, 09:30 authored by Eleanor Woodward, Minjun YangMinjun Yang, Larissa Helena Moura-Castro, Hilda van den Bos, Rebeqa GunnarssonRebeqa Gunnarsson, Linda Olsson Arvidsson, Diana C. J. Spierings, Anders Castor, Nicolas Duployez, Marketa Zaliova, Jan Zuna, Bertil Johansson, Floris Foijer, Kajsa Paulsson


This dataset included 577 samples with high hyperdiploid acute lymphoblastic leukemia (ALL) that were collected from four different cohorts: the Division of Clinical Genetics, Lund University, Sweden, Department of Pediatric Hematology and Oncology, Second Faculty of Medicine, Charles University/University Hospital Motol, Prague, Czech Republic (Zaliova et al., 2016), Laboratory of Hematology, Centre Hospitalier Universitaire (CHU) Lille, Lille, France (Duployez et al., 2018), and The Therapeutically Applicable Research to Generate Effective Treatments (TARGET) program (NCBI dbGAP accession number phs00464). All samples were genotyped using either the Affymetrix SNP Array, Illumina's BeadArray platform or the whole genome/exome sequencing by the Illumina platform.  Nine high hyperdiploid ALL samples and one normal bone marrow sample were subjected to low pass single cell whole genome sequencing with the median sequencing coverage of 0.02x. Single nuclei in G0/G1 phase were isolated using a fluorescence-activated cell sorting (FACS) cytometer. DNA libraries were constructed and associated next-generation sequencing was carried out by European Research Institute for the Biology of Ageing (ERIBA), University of Groningen, University Medical Center Groningen, Groningen, The Netherlands. Further details regarding the DNA libraries construction are available by Bos et. al., 2019 ( The dataset has been used for copy number aberrations analysis. The raw data file can be made available after applying for access and agreeing to the terms and conditions for use.


The role of CTCF and cohesin in leukemia development

Swedish Research Council

Find out more...

Swedish Childhood Cancer Foundation(PR2018-0023, PR2020-0033, and TJ2020-0024)

Swedish Cancer Fund (19-0252-Pj)

Governmental funding of clinical research within the National Health Service (ALFSKANE-623431)

Gunnar Nilsson Cancer Foundation (GN-2020-9 -(190))

The Royal Physiographic Society of Lund

Genetic and epigenetic studies of pediatric acute leukemia

Swedish Research Council

Find out more...



Lund University

Access request email

Usage metrics

    Kajsa Paulsson Lab



    Ref. manager