SARS-CoV-2 Wastewater Data from Stockholm, Sweden

<div><div><p> </p><p>The dataset is part of a research study lead by associate professor Zeynep Cetecioglu Gurol and colleagues (KTH Royal Institute of Technology), is a collaboration between the SciLifeLab COVID-19 National Research Program and Chemical Engineering and Protein Science departments at KTH, in close collaboration with Stockholm Vatten och Avfall and the Käppala Association.</p><p><br></p><p>The sampling of wastewater, started in mid-April 2020, from Bromma, Henriksdal, and Käppala wastewater treatment plants (WWTP). These treatment plants receive wastewater from a population of approximately 360,000; 860,000 and 500,000, respectively.</p><p>After concentration, filtering, and preparation, the samples are analyzed using RT-qPCR technique for SARS CoV-2 RNA. Primers of the nucleocapsid (N) gene were used to detect the SARS-COV-2 gene (previously used and verified by <a href="https://doi.org/10.1016/j.scitotenv.2020.142939">Medema and colleagues (2020)</a>. In some cases, the raw wastewater has been frozen at –20 degrees, and concentrated wastewater or purified RNA have been stored at -80 C before the next analysis step was carried out. The concentration method used by prof. Zeynep Cetecioglu Gurol and her colleagues is based on their published study (<a href="https://doi.org/10.1016/j.scitotenv.2020.142939">Jafferali and colleagues, 2021</a>). The gene copy nr / week is presented standardized for Bovine coronavirus (BCoV) and Pepper Mild Mottle Virus (PPMoV)</p><p>For more information see <a href="https://www.covid19dataportal.se/data_types/environment/wastewater/">https://www.covid19dataportal.se/data_types/environment/wastewater/</a> The dataset started week 16 2020 and updates weekly. </p><p> </p><p>The dataset is available as part of the Environmental Virus Profiling data section "The amount of SARS-CoV-2 virus in wastewater across Sweden" <a href="https://www.covid19dataportal.se/data_types/environment/wastewater/#stockholm">https://www.covid19dataportal.se/data_types/environment/wastewater/#stockholm</a></p><p>on the Swedish COVID-19 Data Portal (<a href="https://covid19dataportal.se">https://covid19dataportal.se</a> ). </p></div></div>