Modulation of innate immune response to mRNA vaccination after SARS-CoV-2 infection or sequential vaccination in humans

### General information

This is a dataset record for the research paper "Modulation of innate immune response to mRNA vaccination after SARS-CoV-2 infection or sequential vaccination in humans" led by professor Karin Loré (Karolinska Institutet) and her research group.

Author(s): Hellgren F, Rosdahl A, Arcoverde Cerveira R, Lenart K, Ols S, Gwon Y-D, Joas G, Kurt S, Delis A-M, Evander M, Normark J, Ahlm C, Forsell M, Cajander S, Loré K

Corresponding author: Karin Loré, Division of Immunology and Allergy, Department of Medicine Solna, Karolinska Institutet, Visionsgatan 4, BioClinicum J7:30, Karolinska University Hospital, 171 64 Stockholm, Sweden.

Contact e-mail: karin.lore@ki.se

Github code repo: https://github.com/rodrigarc/orebro_study/

DOI: https://doi.org/10.1172/jci.insight.175401

This readme file was last updated: 2024-04-22

The dataset is available upon reasonable request through the corresponding author.

### Cohort description

The repository contains metadata for the 30 study participants recruited among health-care workers at the University hospital of Örebro, Sweden. At the start of the study, 14 individuals had a previous Covid-19 infection and 16 were infection naïve. Among the infection naïve group, 75% were female (12 out of 16 participants), with a mean age of 41 years, ranging from 25 to 66 years. In the group with previous Covid-19 infection, 71.4% were female (10 out of 14 participants), with a mean age of 44.6 years, spanning from 29 to 63 years. Study participants were sampled adjacent to each vaccine dose according to the schedule shown in Fig. 1A.

For a more detailed overview of the baseline characteristics please see Table 1 attached in the manuscript.

### Dataset description

Antibody titers measured by ELISA, Percentages of Immunophenotyping of studied cell subsets, and serum protein measurements were compiled into Excel sheets and fully anonymized. This data was provided as supplemental material with the original article.

RNA-sequencing data: RNA-seq analysis of 99 samples was performed using Illumina sequencing. Preprocessing of FASTQ raw reads was done with the nf-core/rnaseq v3.8 pipeline, with results saved in TSV format. The human genome was appended with vaccine and SARS-CoV-2 related genes prior to read alignment using STAR and gene expression quantification with Salmon.

Keywords: mRNA vaccines, innate immunity, Covid-19, coronavirus, vaccine