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Microscopy data set of mRNA FISH and Protein gene expression measurements in which repressor binding strength is systematically altered.

dataset
posted on 2025-01-02, 08:15 authored by Vinodh KandavalliVinodh Kandavalli, Spartak Zikrin, Johan Elf, Daniel Jones

This dataset contains the raw data, analysis and code needed to generate the figures presented in the paper "Anti-correlation of LacI association and dissociation rates observed in living cells" (see Related Material). The raw data consists of microscopy images and qPCR files. The analysis and code consist of data analysis, output from the analysis, its post-processing, simulations, and which figure the data was used in.

Experimental data, and analysis scripts are all organized using unique IDs (UID; seen for example in the filenames of experimental data below).

The README.txt file in the Analysis_of_data folder describes which UIDs for data, analysis, code & figure generation to combine for specific figures.

For the microscopy experiments (has the prefix Microscopy_Data_ in their filenames) there is information on the growth condition and which figure the data was used in. All experiments were performed at 30 degrees Celsius.

Details on the output from microscopy experiments can be found in the file MicroscopyInformation.txt.

Funding

A double-edged sword? Quantifying tradeoffs in CRISPR immunity

Swedish Research Council

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Biophysics in gene regulation - A genome wide approach

European Research Council

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The physics of genetic information processing

Swedish Research Council

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A genome wide approach to replication initiation

Swedish Research Council

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History

Publisher

Uppsala University

SciLifeLab acknowledgement

  • SciLifeLab Data Centre