This dataset refers to the 16S rRNA gene long-read sequencing data described in the article "Full-length multiplexed 16S rRNA gene sequencing works well in self-collected decade old cervicovaginal fluid on paper cards" (unpublished).
DNA was extracted from two 3 mm punches of paper cards containing dried cervicovaginal fluids that had been stored for up to eleven years at room temperature. Full-length 16S rRNA gene amplicons (~1.5 kb) were generated using the Kinnex 16S protocol followed by long-read sequencing using the Pacific Biosciences (PacBio) Revio system. The raw fastq files consist of de-multiplexed and circular consensus sequencing reads. A more detailed description of the methods can be found in the paper.
Funding
The Swedish Cancer Foundation (SE 220604FE, SE 232874PJ, KSu CAN 2018/384)
The Swedish Research Council (SE 2022-00857)
Lena Wäpplings Stiftelse
Erik, Göran och Selanders Stiftelse
The Swedish state under the agreement between the Swedish government and the county council, the ALF-agreement (KSu)