Data from: A systems biology approach identifies B cell maturation antigen (BCMA) as biomarker reflecting oral vaccine induced IgA antibody responses in humans

In this study, RNA-Seq was performed on peripheral blood mononuclear cells (PBMCs) isolated from six volunteers of a oral cholera Dukoral^® vaccine study performed by Mottram et al., 2020.(10.1016/j.vaccine.2019.10.050).

The raw FASQ and indexed aligned BAM files described here were taken from four strong and two non IgA antibody responders of this Dukoral^® vaccine study and are part of another study described by Mottram et al (2021) (Frontiers in Immunology, accepted for publication).

Total RNA-Seq on these PMBCs was performed by GeneCore SU, University of Gothenburg, Sahlgrenska Academy, Gothenburg, Sweden (http://www.cgg.gu.se/genecore-su), using the Illumina Nextseq 500 platform (Illumina, San Diego, CA, USA). cDNA libraries were prepared from total RNA samples (≤1500ng), using the TruSeq Stranded Total RNA Sample Preparation Kit with Ribo-zero Gold (Illumina Inc). Each library was paired-end sequenced (2×75 bp) using the Illumina Nextseq500 Kit High Output V2.

All sequencing reads were analysed for quality control using the prinseq/0.20.3. Quality filtered reads were aligned to the Ensembl human reference genome GRCh38.90 using star/2.5.2b. Read counts mapped towards annotated features were calculated using the HTSeq-count/0.6.1p1 tool. Statistical analysis was performed using DESeq2_1.14.1 within R/3.3 software, applying a significance threshold padj < 0.05.

The oral cholera Dukoral^® vaccine study was performed in accordance with the declaration of Helsinki and Good Clinical practice guidelines. Dukoral^® vaccinated volunteers provided their written informed consent to participate in these studies, including the RNA-seq of their PBMCs. Consequently, the raw RNA datasets are not publicly available. However, controlled access to these FASQ and BAM files can be made available after applying for access and agreeing to terms and conditions before use.