<p>IPSC lines established from fibroblasts of both sibling (MW1=26, MW2=27) and two healthy donors (Ctl2=K02 and Ctl8=K08).</p>
<p><br></p>
<p>Neurocortical differentiation of iPSCs (samples IPS) was carried out as described using Dual-SMAD inhibition that promotes conversion into neural stem cells (NSC) for 10 days (samples INT) followed by directed differentiation into cortical lineages for 65 days (samples D65).</p>
<p><br></p>
<p>Bulk RNA sequencing reads were aligned to the ENSEMBL human reference genome (Homo_sapiens.GRCh37.75) and gene counts were generated using the STAR read aligner. The count data is not normalized and can be used with DESEq2.</p>
<p><br></p>
<p>Expression data was generated for all three time points IPS -> INT (D10) -> D65</p>
<p><br></p>
<p>File names are:</p>
<p>timepoint_SampleReadsPerGene.out.tab</p>
Funding
Hjärnfonden (FO2019-0210 and FO2020-0171)
Borgström Foundation
AstraZeneca
Neurodevelopmental disorders: Identification of shared molecular and cellular mechanisms for intervention and prevention