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Asymmetric nucleosome PARylation at DNA breaks mediates directional nucleosome sliding by ALC1

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posted on 2023-12-19, 11:28 authored by Anton SabantcevAnton Sabantcev, Luka Bacic, Guillaume Gaullier, Guanzhong Mao, Klaus Brackmann, Mikhail PanfilovMikhail Panfilov, Jugal Mohapatra, Glen Liszczak, Sebastian Deindl

Single-molecule Förster resonance energy transfer (FRET) data supporting the findings of the paper titled "Asymmetric nucleosome PARylation at DNA breaks mediates directional nucleosome sliding by ALC1" in Nature Communications. The data mainly pertain to the remodeling directionality of ALC1 as a function of the PARylation state of a nucleosome.

The initial remodeling directionality was measured based on the direction of the initial FRET change upon remodeling. The biotinylated FRET-labeled nucleosomes were immobilized on a PEG (poly[ethylene glycol])-coated quartz slide saturated with streptavidin. Cy3 and Cy5 fluorophores were excited with 532 nm Nd:YAG and 638 nm diode lasers, respectively, and fluorescence emissions from Cy3 and Cy5 fluorophores were detected using a custom-built prism-based TIRF microscope. To check the presence of an intact donor fluorophore, the sample was alternately excited with 532 nm and 638 nm lasers during the experiment.

Funding

Welch Foundation I-2039-2020040

Single-Molecule And Structural Studies Of ATP-Dependent Chromatin Remodelling

European Research Council

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Knut and Alice Wallenberg Foundation 019.0306

Function and regulation of an oncogenic remodeler

Swedish Research Council

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Cancerfonden 19 0055 Pj

Regulation and function of site-specific protein poly-ADP-ribosylation

National Institute of General Medical Sciences

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New technologies to study unique PARP activities in physiology and disease

American Heart Association

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Recruitment of First-Time, Tenure-Track Faculty Members

Cancer Prevention and Research Institute of Texas

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History

Publisher

Uppsala University

SciLifeLab acknowledgement

  • Cryo-EM unit