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Archaic humans have contributed to large-scale variation in modern human T cell receptor genes

posted on 2023-03-17, 10:37 authored by Martin Corcoran, Mark Chernyshev, Marco Mandolesi, Sanjana Narang, Mateusz KadukMateusz Kaduk, Christopher Sundling, Anna Färnert, Carolina Bernhardsson, Maximilian Larena, Mattias Jakobsson, Gunilla B. Karlsson Hedestam

The dataset contains the repertoire sequencing data from 45 adult volunteers.

For each case 4 separate TCR repseq libraries were constructed after cDNA synthesis using a UMI containing primer located in the constant exon of the TRA, TRD, TRB and TRG genes respectively. In each case a 5’ multiplex set of primers specific for the leader regions of the V genes of either TRA, TRD, TRB or TRG genes was used along with a universal reverse primer, introduced during the cDNA synthesis step to produce the gene specific repseq library.

The libraries were sequenced using the Illumina 2 x 300 cycle MiSeq system at the Karlsson Hedestam Laboratory at the Department of Microbiology, Tumor and Cell Biology at Biomedicum, Karolinska Institutet, Stockholm.

Terms for access

The datasets are only to be used for research that is seeking to advance the understanding of the influence of TCR genetic variation on human biology.


ERC and the Swedish Research Council



Karolinska Institutet

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