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A Multi-Parametric and High-Throughput Platform for Host-Virus Binding Screens

dataset
posted on 2023-04-04, 11:00 authored by Erdinc SezginErdinc Sezgin, Jan SchlegelJan Schlegel, Bartlomiej Porebski, Luca Andronico, Leo Hanke, Steven EdwardsSteven Edwards, Hjalmar BrismarHjalmar Brismar, Ben Murrell, Gerald M. McInerney, Oscar Fernández-Capetillo

General information


This item containst data sets for Schlegel et al, Nano Letters, 2023. 

DOI: https://doi.org/10.1021/acs.nanolett.2c04884


It contains confocal images, lattice light sheet images, flow cytometry data, compiled data as excle sheet and raw figure files. 


Abstract

Speed is key during infectious disease outbreaks. It

is essential, for example, to identify critical host binding factors to

pathogens as fast as possible. The complexity of host plasma

membrane is often a limiting factor hindering fast and accurate

determination of host binding factors as well as high-throughput

screening for neutralizing antimicrobial drug targets. Here, we

describe a multiparametric and high-throughput platform tackling

this bottleneck and enabling fast screens for host binding factors as

well as new antiviral drug targets. The sensitivity and robustness of

our platform were validated by blocking SARS-CoV-2 particles

with nanobodies and IgGs from human serum samples.


Data usage

Researchers are welcome to use the data contained in the dataset for any projects. Please cite this item upon use or when published. We encourage reuse using the same CC BY 4.0 License.

 

Data Content

Excel files for graphs

Microscopy Images

Flow cytometry data 


Software to open files:

.csv: Fiji (https://imagej.net/software/fiji/downloads) or Microsoft Excel 

.xlsx: Microsoft Excel 

.tif, .lsm: Fiji (https://imagej.net/software/fiji/downloads)

.pzfx: GraphPad Prism

.svg: Inkscape (https://inkscape.org/)

.fcs: FCS Express

.pdf: AdobeAcrobat or Mozilla Firefox

.ijm: Fiji (https://imagej.net/software/fiji/downloads)

Funding

SciLifeLab

Karolinska Institutet

Knut and Alice Wallenberg foundation

Swedish Research Council

Human Frontier Science Program HFSP

Federal Department of Economic Affairs Education and Research

Find out more...

Marie Skłodowska-Curie fellowship

History

Publisher

Karolinska Institutet

Contact email

erdinc.sezgin@ki.se

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